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mus musculus hepatocyte cell line  (ATCC)


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    ATCC mus musculus hepatocyte cell line
    MMP-9 overexpression decreases IL-6 secretion in primary <t>hepatocytes</t> after LPS induction. Forward transfection of the pCMV6-mMMP-9 plasmid was performed into primary hepatocytes for 24 h, following LPS induction for another 24 h. (A) MMP-9 protein levels in cell lysates were assessed by immunoblotting. GAPDH was used as an internal control. (B) ELISA analyzed the IL-6 secretion in conditioned medium samples. Data are presented as mean ± SEM. n = 8. Different letters represent significant differences determined by one-way ANOVA following Turkey’s multiple comparison. p < 0.05.
    Mus Musculus Hepatocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1542 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mus musculus hepatocyte cell line/product/ATCC
    Average 99 stars, based on 1542 article reviews
    mus musculus hepatocyte cell line - by Bioz Stars, 2026-02
    99/100 stars

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    1) Product Images from "The hemopexin domain of matrix metalloproteinase-9 attenuates lipopolysaccharide-induced interleukin-6 secretion in liver"

    Article Title: The hemopexin domain of matrix metalloproteinase-9 attenuates lipopolysaccharide-induced interleukin-6 secretion in liver

    Journal: Scientific Reports

    doi: 10.1038/s41598-025-32379-y

    MMP-9 overexpression decreases IL-6 secretion in primary hepatocytes after LPS induction. Forward transfection of the pCMV6-mMMP-9 plasmid was performed into primary hepatocytes for 24 h, following LPS induction for another 24 h. (A) MMP-9 protein levels in cell lysates were assessed by immunoblotting. GAPDH was used as an internal control. (B) ELISA analyzed the IL-6 secretion in conditioned medium samples. Data are presented as mean ± SEM. n = 8. Different letters represent significant differences determined by one-way ANOVA following Turkey’s multiple comparison. p < 0.05.
    Figure Legend Snippet: MMP-9 overexpression decreases IL-6 secretion in primary hepatocytes after LPS induction. Forward transfection of the pCMV6-mMMP-9 plasmid was performed into primary hepatocytes for 24 h, following LPS induction for another 24 h. (A) MMP-9 protein levels in cell lysates were assessed by immunoblotting. GAPDH was used as an internal control. (B) ELISA analyzed the IL-6 secretion in conditioned medium samples. Data are presented as mean ± SEM. n = 8. Different letters represent significant differences determined by one-way ANOVA following Turkey’s multiple comparison. p < 0.05.

    Techniques Used: Over Expression, Transfection, Plasmid Preparation, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Comparison



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    MMP-9 overexpression decreases IL-6 secretion in primary hepatocytes after LPS induction. Forward transfection of the pCMV6-mMMP-9 plasmid was performed into primary hepatocytes for 24 h, following LPS induction for another 24 h. (A) MMP-9 protein levels in cell lysates were assessed by immunoblotting. GAPDH was used as an internal control. (B) ELISA analyzed the IL-6 secretion in conditioned medium samples. Data are presented as mean ± SEM. n = 8. Different letters represent significant differences determined by one-way ANOVA following Turkey’s multiple comparison. p < 0.05.

    Journal: Scientific Reports

    Article Title: The hemopexin domain of matrix metalloproteinase-9 attenuates lipopolysaccharide-induced interleukin-6 secretion in liver

    doi: 10.1038/s41598-025-32379-y

    Figure Lengend Snippet: MMP-9 overexpression decreases IL-6 secretion in primary hepatocytes after LPS induction. Forward transfection of the pCMV6-mMMP-9 plasmid was performed into primary hepatocytes for 24 h, following LPS induction for another 24 h. (A) MMP-9 protein levels in cell lysates were assessed by immunoblotting. GAPDH was used as an internal control. (B) ELISA analyzed the IL-6 secretion in conditioned medium samples. Data are presented as mean ± SEM. n = 8. Different letters represent significant differences determined by one-way ANOVA following Turkey’s multiple comparison. p < 0.05.

    Article Snippet: RAW264.7, a Mus musculus macrophage cell line (ATCC ® TIB-71TM), Hepa1-6, a Mus musculus hepatocyte cell line (ATCC ® CRL-1830TM), and 293T, a Homo sapiens embryonic kidney cell line (ATCC ® CRL-3216TM), were cultured in DMEM with high glucose medium (Gibco) supplemented with 10% fetal bovine serum, 1% Penicillin-Streptomycin, 3.7 mg/L NaHCO 3 , and 1 mM sodium pyruvate and disinfected with a 0.22 μm filter.

    Techniques: Over Expression, Transfection, Plasmid Preparation, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Comparison